1. Introduction

Pure goat milk is a high-end dairy product. It contains small fat globules and is easily absorbed, making it suitable for people with lactose intolerance, the elderly, and infants. It is also rich in whey protein, immunoglobulins, and various nutrients, with a high proportion of unsaturated fatty acids.
Due to limited goat milk production, a significant gap exists between supply and demand. As a result, goat milk is sometimes adulterated with cow milk to reduce production costs. The frequent occurrence of such adulteration poses health risks to consumers, particularly those allergic to cow milk. Allergic reactions may include skin itching, redness and swelling, nausea, and vomiting, and in severe cases may lead to laryngeal edema, breathing difficulties, or anaphylactic shock. In addition, enterprises may face consumer disputes due to substandard products, resulting in economic losses and damage to brand reputation.

2-Product Principles and Advantages

Detection Principle

Bispecific antibody sandwich method: A conjugate of anti-α-lactalbumin monoclonal antibody 1 (Ab-1) and colloidal gold-labeled monoclonal antibody 2 (Ab-2-Au) is used to bind different epitopes of the target antigen. Through this specific antigen–antibody reaction, qualitative detection of the target protein is achieved.

Product Features

1）Strong specificity:
Anti-α-lactalbumin monoclonal antibody 1 specifically recognizes α-lactalbumin in cow’s milk and shows no cross-reactivity with other common food matrices.

2）High sensitivity:
The detection sensitivity is 1%. A positive result can be obtained when 1 mL of cow’s milk is present in 100 mL of goat milk (equivalent to a 1:100 dilution).

3）Convenient and efficient operation:
Sample pretreatment requires only simple dilution. Testing can be performed directly, and results can be read by the naked eye within 10 minutes.

4）Application scenarios:
Suitable for raw material quality control in goat milk processing plants, inspection of finished products at milk stations, pastures, and distribution points, as well as product quality spot checks by regulatory authorities.

3-Product Parameter

Product name	PriboStripTM Goat Milk Adulterated By Cow Milk Rapid Test Strips
Product number	PRS-F010
Format	10T/20T
Detection object	α -lactalbumin in milk
Sensitivity	1 %
Testing time	10 min
Applicable substrate	Goat milk, goat milk powder

4-Method

1. Before use, allow the test strip and the sample solution to reach room temperature.
2. Dilute the goat milk or goat milk powder 100-fold with the sample diluent to obtain the test solution.
3. Pipette 200 µL of the test solution into a centrifuge tube. Insert the test strip directly into the tube. Ensure that the liquid level does not exceed the arrow mark on the strip. Interpret the result after 10 minutes.

5-Results Analysis

1. Negative result: Only the C line of the test strip is colored.
2. Positive result: Both C and T lines are colored.
3. Invalid result: When the C line of the test strip is not colored, the reaction fails. Please check the following possible reasons, and retest with a new test strip:

A: If the test strip packaging is damaged.

B: If the test strip is damp.

C: If the sample is too thick or too concentrated.

6-Allergen product series

Other milk testing related products are as follows:

Product number	Product name	Detection object	Format
PRS-A60	PriboStripTM Casein (Bovine milk) Rapid Test Strip	Casein	10T/20T
PRS-A60-JT	PriboStripTM Casein (Bovine milk) Rapid Test Strip Family Pack	Casein	2T
EKT-L60	PriboFast® β-Lactoglobulin (Bovine milk) ELISA Kit	β-Lactoglobulin	48T/96T
EKT-CA61	PriboFast® Casein (Bovine milk) ELISA Kit	Casein	48T/96T
EKT-ML60	PriboFast® Milk (Casein/β-Lactoglobulin) ELISA Kit	Casein/β-Lactoglobulin	48T/96T
EKT-LB10	PriboFast® α-Lactalbumin ELISA Kit	α-Lactalbumin	48T/96T

Qingdao PriboStrip™ offers a full range of rapid food allergen detection platforms, providing a wide variety of allergen detection products to help enterprises effectively control the risk of allergen cross-contamination. The available products include:
• The eight major allergens: peanuts, soybeans, eggs, gluten, crustaceans, fish, milk, nuts (such as almonds, hazelnuts), etc.

• Other common allergens: sesame, buckwheat, bitter buckwheat, lysozyme and histamine, etc.

• Vegetable series: Lupin beans, celery, etc.

• Condiment series: Mustard.

7-Company profile

Qingdao Puribang Bioengineering Co., Ltd. was established in 2008 and specializes in the research and application of food testing products. After more than ten years of development, the company has established a comprehensive product system covering biological toxin reference materials, stable isotope internal standards, quality control samples for various matrices, sample pretreatment consumables, rapid detection products, and intelligent sample pretreatment instruments, and has developed a number of proprietary products.

Contact number: 400-6885349

• mail: into@pribolab.com

Website: www.pribolab.com